php eb aav syn jgcamp7s wpre Search Results


paav syn dio jgcamp7s  (Addgene inc)
95
Addgene inc paav syn dio jgcamp7s
a , Experimental diagram of fiber photometry and optogenetic stimulation. ChrimsonR-expressing CST axons were optically stimulated and calcium responses of <t>jGCaMP7s-expressing</t> Chx10 + interneurons were recorded at C5 and C7. Bottom, experimental timeline. b , Representative images of jGCaMP7s (green) and ChrimsonR-tdTomato expression (red) in the cervical cord at day 28 post-stroke. Bottom, a magnified view of the dotted area. Scale bars, 100 µm. c , Representative optically-evoked calcium responses of V2a interneurons at C5 (left) and C7 (right) levels in control (black) and stroke mice at day 28 (blue) and 56 (red). The solid lines and shaded areas represent the average and standard deviation of fluorescence changes, respectively. d , Peak amplitude of calcium responses. N = 6, 5 and 7 mice for control, day 28, and day 56, respectively; one-way ANOVA followed by Tukey’s test, *** p < 0.001 and **** p < 0.0001. e , Representative kymographs of calcium responses evoked by optical stimulation at C5 and C7 in controls and at day 28 and 56 post-stroke (50 trials/one mouse). The green bars and the dotted lines indicate the timing of photostimulation.
Paav Syn Dio Jgcamp7s, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/php+eb+aav+syn+jgcamp7s+wpre/bio_rxiv__2025__01__21__634186-229-13-24?v=Addgene+inc
Average 95 stars, based on 1 article reviews
paav syn dio jgcamp7s - by Bioz Stars, 2026-08
95/100 stars
  Buy from Supplier
pgp aav syn flex jgcamp7s wpre  (Addgene inc)
94
Addgene inc pgp aav syn flex jgcamp7s wpre
a , Experimental diagram of fiber photometry and optogenetic stimulation. ChrimsonR-expressing CST axons were optically stimulated and calcium responses of <t>jGCaMP7s-expressing</t> Chx10 + interneurons were recorded at C5 and C7. Bottom, experimental timeline. b , Representative images of jGCaMP7s (green) and ChrimsonR-tdTomato expression (red) in the cervical cord at day 28 post-stroke. Bottom, a magnified view of the dotted area. Scale bars, 100 µm. c , Representative optically-evoked calcium responses of V2a interneurons at C5 (left) and C7 (right) levels in control (black) and stroke mice at day 28 (blue) and 56 (red). The solid lines and shaded areas represent the average and standard deviation of fluorescence changes, respectively. d , Peak amplitude of calcium responses. N = 6, 5 and 7 mice for control, day 28, and day 56, respectively; one-way ANOVA followed by Tukey’s test, *** p < 0.001 and **** p < 0.0001. e , Representative kymographs of calcium responses evoked by optical stimulation at C5 and C7 in controls and at day 28 and 56 post-stroke (50 trials/one mouse). The green bars and the dotted lines indicate the timing of photostimulation.
Pgp Aav Syn Flex Jgcamp7s Wpre, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/php+eb+aav+syn+jgcamp7s+wpre/pm37632130-69-8-9?v=Addgene+inc
Average 94 stars, based on 1 article reviews
pgp aav syn flex jgcamp7s wpre - by Bioz Stars, 2026-08
94/100 stars
  Buy from Supplier
gcamp7s transgenes  (Addgene inc)
96
Addgene inc gcamp7s transgenes
A) Adult born immature granule cells were silenced using designer receptor exclusively activated by designer drugs (hM4Di), which activates inhibitory Gi-signaling upon stimulation by the ligand, clozapine-N-oxide (CNO). Tamoxifen (TAM) inducible Ascl CreER ;Di F/F mice and Di F/F controls were injected with Tamoxifen (100mg/kg) for 5 consecutive days, and followed by rAAV injection and window implantation 2 weeks later. Head-fixed mice were subsequently trained to run on a featureless belt to find randomly scattered hidden rewards. (Bottom) Mice were tested in different Contexts as in but different olfactory stimuli were delivered in the middle of one of the belt sections (1sec.). First session in context A was imaged as a baseline, then all mice received CNO (5mg/kg) i . p . injections 30 min before running in contexts A’ and B while at home cage. B) A separate cohort of Ascl CreER ;TdTomato F/F mice were injected with TAM followed by <t>AAV-GCaMp7s</t> injection 2 weeks later. AAV injection did not result in differences in genetically targeted iGCs in the injected hemisphere compared to the contralateral hemisphere, for quantification see . C) Spatial firing patterns during 3 sequential exposures to the same and different contexts in Di F/F control mice. The second exposure to Context A is shown as A’. Cells selected based on having a place field in at least one session and are arranged based on maximum events rate in A. Each row across all graphs represents a single cell matched across all contexts. N Cre- =261 cells, 7 mice. D) Spatial firing patterns during 3 sequential exposures to the same and different contexts in Ascl CreER ;Di F/F mice. N Cre+ = 275 cells, 6 mice. E) Top: Similarity of spatial representations of cells with a field in at least one context are measured by calculating Pearson’s correlations of rate maps between sequential exposures to the same (A-A’) or different (A’-B) contexts (top). Bottom: Bar plots represent group data; circles represent per animal data. Interaction F 1,22 = 11.15, ** P =0.0030; Context F 1,22 = 191.9, *** P <0.0001; Genotype F 1,22 = 34.89, ** P <0.0001; 2-way ANOVA. Same context: P Cre- – Cre + = 0.2928; Different context: *** P Cre- – Cre + <0.0001; Tukey’s multiple comparisons test. F) Position decoded by a Bayesian algorithm among cells found in all sessions by training using neural activity in A’ and calculating predicted position in the same (A) or different (B) contexts (i). Line graphs represent median spatial decoder error calculated as the difference between the predicted position in the training data from those in test datasets that is higher in Cre negative Di F/F animals compared to Ascl CreER ;Di F/F when spatial activity from different environments was used to decode position (ii). Di F/F : W = 28, * P Same Ctx – Different Ctx = 0.0156; Ascl CreER ;Di F/F : W = 15, P Same Ctx – Different Ctx = 0.1563; Wilcoxon rank sum test. iii. Discrimination index was calculated as: (Error Different Context - Error Same Context )/ (Error Different Context + Error Same Context ), U =4, * P Cre—Cre+ = 0 . 0 140, Mann-Whitney test. Di F/F : 83.42 ± 5.89, Ascl CreER ;Di F/F : 83 ± 8.68 granule cells matched in test and train sessions. Error bars, ± sem. ( iii, see also )
Gcamp7s Transgenes, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/php+eb+aav+syn+jgcamp7s+wpre/bio_rxiv__2022__12__08__519632-171-13-20?v=Addgene+inc
Average 96 stars, based on 1 article reviews
gcamp7s transgenes - by Bioz Stars, 2026-08
96/100 stars
  Buy from Supplier
noti  (New England Biolabs)
99
New England Biolabs noti
A) Adult born immature granule cells were silenced using designer receptor exclusively activated by designer drugs (hM4Di), which activates inhibitory Gi-signaling upon stimulation by the ligand, clozapine-N-oxide (CNO). Tamoxifen (TAM) inducible Ascl CreER ;Di F/F mice and Di F/F controls were injected with Tamoxifen (100mg/kg) for 5 consecutive days, and followed by rAAV injection and window implantation 2 weeks later. Head-fixed mice were subsequently trained to run on a featureless belt to find randomly scattered hidden rewards. (Bottom) Mice were tested in different Contexts as in but different olfactory stimuli were delivered in the middle of one of the belt sections (1sec.). First session in context A was imaged as a baseline, then all mice received CNO (5mg/kg) i . p . injections 30 min before running in contexts A’ and B while at home cage. B) A separate cohort of Ascl CreER ;TdTomato F/F mice were injected with TAM followed by <t>AAV-GCaMp7s</t> injection 2 weeks later. AAV injection did not result in differences in genetically targeted iGCs in the injected hemisphere compared to the contralateral hemisphere, for quantification see . C) Spatial firing patterns during 3 sequential exposures to the same and different contexts in Di F/F control mice. The second exposure to Context A is shown as A’. Cells selected based on having a place field in at least one session and are arranged based on maximum events rate in A. Each row across all graphs represents a single cell matched across all contexts. N Cre- =261 cells, 7 mice. D) Spatial firing patterns during 3 sequential exposures to the same and different contexts in Ascl CreER ;Di F/F mice. N Cre+ = 275 cells, 6 mice. E) Top: Similarity of spatial representations of cells with a field in at least one context are measured by calculating Pearson’s correlations of rate maps between sequential exposures to the same (A-A’) or different (A’-B) contexts (top). Bottom: Bar plots represent group data; circles represent per animal data. Interaction F 1,22 = 11.15, ** P =0.0030; Context F 1,22 = 191.9, *** P <0.0001; Genotype F 1,22 = 34.89, ** P <0.0001; 2-way ANOVA. Same context: P Cre- – Cre + = 0.2928; Different context: *** P Cre- – Cre + <0.0001; Tukey’s multiple comparisons test. F) Position decoded by a Bayesian algorithm among cells found in all sessions by training using neural activity in A’ and calculating predicted position in the same (A) or different (B) contexts (i). Line graphs represent median spatial decoder error calculated as the difference between the predicted position in the training data from those in test datasets that is higher in Cre negative Di F/F animals compared to Ascl CreER ;Di F/F when spatial activity from different environments was used to decode position (ii). Di F/F : W = 28, * P Same Ctx – Different Ctx = 0.0156; Ascl CreER ;Di F/F : W = 15, P Same Ctx – Different Ctx = 0.1563; Wilcoxon rank sum test. iii. Discrimination index was calculated as: (Error Different Context - Error Same Context )/ (Error Different Context + Error Same Context ), U =4, * P Cre—Cre+ = 0 . 0 140, Mann-Whitney test. Di F/F : 83.42 ± 5.89, Ascl CreER ;Di F/F : 83 ± 8.68 granule cells matched in test and train sessions. Error bars, ± sem. ( iii, see also )
Noti, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/php+eb+aav+syn+jgcamp7s+wpre/pmc12441120-297-33-51?v=New+England+Biolabs
Average 99 stars, based on 1 article reviews
noti - by Bioz Stars, 2026-08
99/100 stars
  Buy from Supplier
aav9 syn flex jgcamp7s wpre  (Vigene Biosciences)
86
Vigene Biosciences aav9 syn flex jgcamp7s wpre
A) Adult born immature granule cells were silenced using designer receptor exclusively activated by designer drugs (hM4Di), which activates inhibitory Gi-signaling upon stimulation by the ligand, clozapine-N-oxide (CNO). Tamoxifen (TAM) inducible Ascl CreER ;Di F/F mice and Di F/F controls were injected with Tamoxifen (100mg/kg) for 5 consecutive days, and followed by rAAV injection and window implantation 2 weeks later. Head-fixed mice were subsequently trained to run on a featureless belt to find randomly scattered hidden rewards. (Bottom) Mice were tested in different Contexts as in but different olfactory stimuli were delivered in the middle of one of the belt sections (1sec.). First session in context A was imaged as a baseline, then all mice received CNO (5mg/kg) i . p . injections 30 min before running in contexts A’ and B while at home cage. B) A separate cohort of Ascl CreER ;TdTomato F/F mice were injected with TAM followed by <t>AAV-GCaMp7s</t> injection 2 weeks later. AAV injection did not result in differences in genetically targeted iGCs in the injected hemisphere compared to the contralateral hemisphere, for quantification see . C) Spatial firing patterns during 3 sequential exposures to the same and different contexts in Di F/F control mice. The second exposure to Context A is shown as A’. Cells selected based on having a place field in at least one session and are arranged based on maximum events rate in A. Each row across all graphs represents a single cell matched across all contexts. N Cre- =261 cells, 7 mice. D) Spatial firing patterns during 3 sequential exposures to the same and different contexts in Ascl CreER ;Di F/F mice. N Cre+ = 275 cells, 6 mice. E) Top: Similarity of spatial representations of cells with a field in at least one context are measured by calculating Pearson’s correlations of rate maps between sequential exposures to the same (A-A’) or different (A’-B) contexts (top). Bottom: Bar plots represent group data; circles represent per animal data. Interaction F 1,22 = 11.15, ** P =0.0030; Context F 1,22 = 191.9, *** P <0.0001; Genotype F 1,22 = 34.89, ** P <0.0001; 2-way ANOVA. Same context: P Cre- – Cre + = 0.2928; Different context: *** P Cre- – Cre + <0.0001; Tukey’s multiple comparisons test. F) Position decoded by a Bayesian algorithm among cells found in all sessions by training using neural activity in A’ and calculating predicted position in the same (A) or different (B) contexts (i). Line graphs represent median spatial decoder error calculated as the difference between the predicted position in the training data from those in test datasets that is higher in Cre negative Di F/F animals compared to Ascl CreER ;Di F/F when spatial activity from different environments was used to decode position (ii). Di F/F : W = 28, * P Same Ctx – Different Ctx = 0.0156; Ascl CreER ;Di F/F : W = 15, P Same Ctx – Different Ctx = 0.1563; Wilcoxon rank sum test. iii. Discrimination index was calculated as: (Error Different Context - Error Same Context )/ (Error Different Context + Error Same Context ), U =4, * P Cre—Cre+ = 0 . 0 140, Mann-Whitney test. Di F/F : 83.42 ± 5.89, Ascl CreER ;Di F/F : 83 ± 8.68 granule cells matched in test and train sessions. Error bars, ± sem. ( iii, see also )
Aav9 Syn Flex Jgcamp7s Wpre, supplied by Vigene Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/php+eb+aav+syn+jgcamp7s+wpre/us12655444-139-20-2?v=Vigene+Biosciences
Average 86 stars, based on 1 article reviews
aav9 syn flex jgcamp7s wpre - by Bioz Stars, 2026-08
86/100 stars
  Buy from Supplier
aav1 syn flex jgcamp7s wpre  (Addgene inc)
95
Addgene inc aav1 syn flex jgcamp7s wpre
A) Adult born immature granule cells were silenced using designer receptor exclusively activated by designer drugs (hM4Di), which activates inhibitory Gi-signaling upon stimulation by the ligand, clozapine-N-oxide (CNO). Tamoxifen (TAM) inducible Ascl CreER ;Di F/F mice and Di F/F controls were injected with Tamoxifen (100mg/kg) for 5 consecutive days, and followed by rAAV injection and window implantation 2 weeks later. Head-fixed mice were subsequently trained to run on a featureless belt to find randomly scattered hidden rewards. (Bottom) Mice were tested in different Contexts as in but different olfactory stimuli were delivered in the middle of one of the belt sections (1sec.). First session in context A was imaged as a baseline, then all mice received CNO (5mg/kg) i . p . injections 30 min before running in contexts A’ and B while at home cage. B) A separate cohort of Ascl CreER ;TdTomato F/F mice were injected with TAM followed by <t>AAV-GCaMp7s</t> injection 2 weeks later. AAV injection did not result in differences in genetically targeted iGCs in the injected hemisphere compared to the contralateral hemisphere, for quantification see . C) Spatial firing patterns during 3 sequential exposures to the same and different contexts in Di F/F control mice. The second exposure to Context A is shown as A’. Cells selected based on having a place field in at least one session and are arranged based on maximum events rate in A. Each row across all graphs represents a single cell matched across all contexts. N Cre- =261 cells, 7 mice. D) Spatial firing patterns during 3 sequential exposures to the same and different contexts in Ascl CreER ;Di F/F mice. N Cre+ = 275 cells, 6 mice. E) Top: Similarity of spatial representations of cells with a field in at least one context are measured by calculating Pearson’s correlations of rate maps between sequential exposures to the same (A-A’) or different (A’-B) contexts (top). Bottom: Bar plots represent group data; circles represent per animal data. Interaction F 1,22 = 11.15, ** P =0.0030; Context F 1,22 = 191.9, *** P <0.0001; Genotype F 1,22 = 34.89, ** P <0.0001; 2-way ANOVA. Same context: P Cre- – Cre + = 0.2928; Different context: *** P Cre- – Cre + <0.0001; Tukey’s multiple comparisons test. F) Position decoded by a Bayesian algorithm among cells found in all sessions by training using neural activity in A’ and calculating predicted position in the same (A) or different (B) contexts (i). Line graphs represent median spatial decoder error calculated as the difference between the predicted position in the training data from those in test datasets that is higher in Cre negative Di F/F animals compared to Ascl CreER ;Di F/F when spatial activity from different environments was used to decode position (ii). Di F/F : W = 28, * P Same Ctx – Different Ctx = 0.0156; Ascl CreER ;Di F/F : W = 15, P Same Ctx – Different Ctx = 0.1563; Wilcoxon rank sum test. iii. Discrimination index was calculated as: (Error Different Context - Error Same Context )/ (Error Different Context + Error Same Context ), U =4, * P Cre—Cre+ = 0 . 0 140, Mann-Whitney test. Di F/F : 83.42 ± 5.89, Ascl CreER ;Di F/F : 83 ± 8.68 granule cells matched in test and train sessions. Error bars, ± sem. ( iii, see also )
Aav1 Syn Flex Jgcamp7s Wpre, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/php+eb+aav+syn+jgcamp7s+wpre/pmc11499281-214-19-20?v=Addgene+inc
Average 95 stars, based on 1 article reviews
aav1 syn flex jgcamp7s wpre - by Bioz Stars, 2026-08
95/100 stars
  Buy from Supplier
aav1  (Addgene inc)
94
Addgene inc aav1
A) Adult born immature granule cells were silenced using designer receptor exclusively activated by designer drugs (hM4Di), which activates inhibitory Gi-signaling upon stimulation by the ligand, clozapine-N-oxide (CNO). Tamoxifen (TAM) inducible Ascl CreER ;Di F/F mice and Di F/F controls were injected with Tamoxifen (100mg/kg) for 5 consecutive days, and followed by rAAV injection and window implantation 2 weeks later. Head-fixed mice were subsequently trained to run on a featureless belt to find randomly scattered hidden rewards. (Bottom) Mice were tested in different Contexts as in but different olfactory stimuli were delivered in the middle of one of the belt sections (1sec.). First session in context A was imaged as a baseline, then all mice received CNO (5mg/kg) i . p . injections 30 min before running in contexts A’ and B while at home cage. B) A separate cohort of Ascl CreER ;TdTomato F/F mice were injected with TAM followed by <t>AAV-GCaMp7s</t> injection 2 weeks later. AAV injection did not result in differences in genetically targeted iGCs in the injected hemisphere compared to the contralateral hemisphere, for quantification see . C) Spatial firing patterns during 3 sequential exposures to the same and different contexts in Di F/F control mice. The second exposure to Context A is shown as A’. Cells selected based on having a place field in at least one session and are arranged based on maximum events rate in A. Each row across all graphs represents a single cell matched across all contexts. N Cre- =261 cells, 7 mice. D) Spatial firing patterns during 3 sequential exposures to the same and different contexts in Ascl CreER ;Di F/F mice. N Cre+ = 275 cells, 6 mice. E) Top: Similarity of spatial representations of cells with a field in at least one context are measured by calculating Pearson’s correlations of rate maps between sequential exposures to the same (A-A’) or different (A’-B) contexts (top). Bottom: Bar plots represent group data; circles represent per animal data. Interaction F 1,22 = 11.15, ** P =0.0030; Context F 1,22 = 191.9, *** P <0.0001; Genotype F 1,22 = 34.89, ** P <0.0001; 2-way ANOVA. Same context: P Cre- – Cre + = 0.2928; Different context: *** P Cre- – Cre + <0.0001; Tukey’s multiple comparisons test. F) Position decoded by a Bayesian algorithm among cells found in all sessions by training using neural activity in A’ and calculating predicted position in the same (A) or different (B) contexts (i). Line graphs represent median spatial decoder error calculated as the difference between the predicted position in the training data from those in test datasets that is higher in Cre negative Di F/F animals compared to Ascl CreER ;Di F/F when spatial activity from different environments was used to decode position (ii). Di F/F : W = 28, * P Same Ctx – Different Ctx = 0.0156; Ascl CreER ;Di F/F : W = 15, P Same Ctx – Different Ctx = 0.1563; Wilcoxon rank sum test. iii. Discrimination index was calculated as: (Error Different Context - Error Same Context )/ (Error Different Context + Error Same Context ), U =4, * P Cre—Cre+ = 0 . 0 140, Mann-Whitney test. Di F/F : 83.42 ± 5.89, Ascl CreER ;Di F/F : 83 ± 8.68 granule cells matched in test and train sessions. Error bars, ± sem. ( iii, see also )
Aav1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/php+eb+aav+syn+jgcamp7s+wpre/10__7554_slash_elife__86022-247-35-31?v=Addgene+inc
Average 94 stars, based on 1 article reviews
aav1 - by Bioz Stars, 2026-08
94/100 stars
  Buy from Supplier
jgcamp7s sequence  (Addgene inc)
94
Addgene inc jgcamp7s sequence
(A) Schematic diagram of viral vector ( <t>AAV-TRE-jGCaMP7s</t> ) injection and optical fiber implantation at the SCN in control ( Avp-Cre; Vgat wt/flox ; Vip-tTA ) or Avp-Vgat −/− ( Avp-Cre; Vgat flox/flox ; Vip-tTA ) mice for fiber photometry recording. (B) A representative coronal section of mice with jGCaMP7s expression in SCN VIP neurons. A white dotted square shows the estimated position of the implanted optical fiber. Green, jGCaMP7s; blue, DAPI. Scale bar, 1 mm. (C) Representative plots of the in vivo jGCaMP7s signal of SCN VIP neurons (green: VIP-Ca 2+ ) overlaid with locomotor activity (black) in actograms. Control (Left) and Avp-Vgat −/− (Right) mice were initially housed in LD (LD1 to LD5), then in DD (DD1 to DD13). Gray shading indicates the time when the lights were off. (D) Plots of locomotor activity onset (black), activity offset (gray), VIP-Ca 2+ onset (green), VIP-Ca 2+ offset (light green), and VIP-Ca 2+ midpoint (magenta) of mean ± SEM (left column) and individual (right column) in control and Avp-Vgat −/− mice. Identical marker shapes indicate data from the same animal. (E) High VIP-Ca 2+ duration in LD (LD1-5, left) or in DD (DD6-10, right). (F) Normalized VIP-Ca 2+ daily rhythm profiles in LD (LD1-5, top) or in DD (DD6-10, bottom). In DD, CT12 was determined as the onset of locomotor activity. Red, Avp-Vgat −/− (n = 8); blue, Control ( Avp-Vgat +/− , i.e., Avp-Cre; Vgat wt/flox , n = 5); Values are mean ± SEM. *P < 0.05 by two-tailed Welch’s t-test.
Jgcamp7s Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/php+eb+aav+syn+jgcamp7s+wpre/bio_rxiv__2025__04__28__650944-142-20-31?v=Addgene+inc
Average 94 stars, based on 1 article reviews
jgcamp7s sequence - by Bioz Stars, 2026-08
94/100 stars
  Buy from Supplier
nir-geco2, nir-geco2g, ho1, cochr, jgcamp7s  (GenScript corporation)
90
GenScript corporation nir-geco2, nir-geco2g, ho1, cochr, jgcamp7s
( a ) Left, fluorescent image of neurons expressing <t>NLS-jGCaMP7s</t> (λ ex = 488-nm laser light, λ em = 527/50 nm). Right, fluorescent image of neurons expressing NIR-GECO2-T2A-HO1 (λ ex = 640-nm laser light, λ em = 685/40 nm). Representative of more than 3 worms, both under tag-168 promoter. Scale bar, 50 μm. ( b ) Fluorescence traces of NLS-jGCaMP7s (top) and NLS-NIR-GECO2 (bottom) in response to the stimulation of microfluidic containing 200 mM NaCl. Solid lines represent averaged data from 3 neurons. Shaded areas are shown as SD. Triangles on the top of the traces indicate the time points of stimulation (20 seconds for each stimulation). ( c ) Quantitative fluorescence changes of NLS-jGCaMP7s and NLS-NIR-GECO2 in b ( n = 36 spikes from 3 neurons). ( d ) Fluorescence image of the 4 C . elegans expressing NIR-GECO2-T2A-HO1 in AVA neurons (under flp-18 promoter) and CoChR-GFP in ASH neurons (under sra-6 promoter). The merged image is shown. Imaging conditions: NIR-GECO2, λ ex = 640-nm laser light, λ em = 685/40; GFP, λ ex = 488-nm laser light, λ ex = 527/50 nm. ( e ) Individual traces of NIR-GECO2 fluorescence in an AVA neuron under blue light illumination (20 mW/mm 2 , λ ex = 488-nm laser light, 100 ms; blue bars). The underlying data for b , c , and e can be found in . NIR, near-infrared; SD, standard deviation.
Nir Geco2, Nir Geco2g, Ho1, Cochr, Jgcamp7s, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/php+eb+aav+syn+jgcamp7s+wpre/pmc07723245-174-8-24?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
nir-geco2, nir-geco2g, ho1, cochr, jgcamp7s - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
raav syn jgcamp7s wpre virus  (Obio Technology Corp Ltd)
86
Obio Technology Corp Ltd raav syn jgcamp7s wpre virus
( a ) Left, fluorescent image of neurons expressing <t>NLS-jGCaMP7s</t> (λ ex = 488-nm laser light, λ em = 527/50 nm). Right, fluorescent image of neurons expressing NIR-GECO2-T2A-HO1 (λ ex = 640-nm laser light, λ em = 685/40 nm). Representative of more than 3 worms, both under tag-168 promoter. Scale bar, 50 μm. ( b ) Fluorescence traces of NLS-jGCaMP7s (top) and NLS-NIR-GECO2 (bottom) in response to the stimulation of microfluidic containing 200 mM NaCl. Solid lines represent averaged data from 3 neurons. Shaded areas are shown as SD. Triangles on the top of the traces indicate the time points of stimulation (20 seconds for each stimulation). ( c ) Quantitative fluorescence changes of NLS-jGCaMP7s and NLS-NIR-GECO2 in b ( n = 36 spikes from 3 neurons). ( d ) Fluorescence image of the 4 C . elegans expressing NIR-GECO2-T2A-HO1 in AVA neurons (under flp-18 promoter) and CoChR-GFP in ASH neurons (under sra-6 promoter). The merged image is shown. Imaging conditions: NIR-GECO2, λ ex = 640-nm laser light, λ em = 685/40; GFP, λ ex = 488-nm laser light, λ ex = 527/50 nm. ( e ) Individual traces of NIR-GECO2 fluorescence in an AVA neuron under blue light illumination (20 mW/mm 2 , λ ex = 488-nm laser light, 100 ms; blue bars). The underlying data for b , c , and e can be found in . NIR, near-infrared; SD, standard deviation.
Raav Syn Jgcamp7s Wpre Virus, supplied by Obio Technology Corp Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/php+eb+aav+syn+jgcamp7s+wpre/pmc12305171-86-4-6?v=Obio+Technology+Corp+Ltd
Average 86 stars, based on 1 article reviews
raav syn jgcamp7s wpre virus - by Bioz Stars, 2026-08
86/100 stars
  Buy from Supplier
raav2 retro jgcamp7s  (Addgene inc)
93
Addgene inc raav2 retro jgcamp7s
( a ) Left, fluorescent image of neurons expressing <t>NLS-jGCaMP7s</t> (λ ex = 488-nm laser light, λ em = 527/50 nm). Right, fluorescent image of neurons expressing NIR-GECO2-T2A-HO1 (λ ex = 640-nm laser light, λ em = 685/40 nm). Representative of more than 3 worms, both under tag-168 promoter. Scale bar, 50 μm. ( b ) Fluorescence traces of NLS-jGCaMP7s (top) and NLS-NIR-GECO2 (bottom) in response to the stimulation of microfluidic containing 200 mM NaCl. Solid lines represent averaged data from 3 neurons. Shaded areas are shown as SD. Triangles on the top of the traces indicate the time points of stimulation (20 seconds for each stimulation). ( c ) Quantitative fluorescence changes of NLS-jGCaMP7s and NLS-NIR-GECO2 in b ( n = 36 spikes from 3 neurons). ( d ) Fluorescence image of the 4 C . elegans expressing NIR-GECO2-T2A-HO1 in AVA neurons (under flp-18 promoter) and CoChR-GFP in ASH neurons (under sra-6 promoter). The merged image is shown. Imaging conditions: NIR-GECO2, λ ex = 640-nm laser light, λ em = 685/40; GFP, λ ex = 488-nm laser light, λ ex = 527/50 nm. ( e ) Individual traces of NIR-GECO2 fluorescence in an AVA neuron under blue light illumination (20 mW/mm 2 , λ ex = 488-nm laser light, 100 ms; blue bars). The underlying data for b , c , and e can be found in . NIR, near-infrared; SD, standard deviation.
Raav2 Retro Jgcamp7s, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/php+eb+aav+syn+jgcamp7s+wpre/pmc08556359-1094-6-10?v=Addgene+inc
Average 93 stars, based on 1 article reviews
raav2 retro jgcamp7s - by Bioz Stars, 2026-08
93/100 stars
  Buy from Supplier
virus  (Addgene inc)
94
Addgene inc virus
( a ) Left, fluorescent image of neurons expressing <t>NLS-jGCaMP7s</t> (λ ex = 488-nm laser light, λ em = 527/50 nm). Right, fluorescent image of neurons expressing NIR-GECO2-T2A-HO1 (λ ex = 640-nm laser light, λ em = 685/40 nm). Representative of more than 3 worms, both under tag-168 promoter. Scale bar, 50 μm. ( b ) Fluorescence traces of NLS-jGCaMP7s (top) and NLS-NIR-GECO2 (bottom) in response to the stimulation of microfluidic containing 200 mM NaCl. Solid lines represent averaged data from 3 neurons. Shaded areas are shown as SD. Triangles on the top of the traces indicate the time points of stimulation (20 seconds for each stimulation). ( c ) Quantitative fluorescence changes of NLS-jGCaMP7s and NLS-NIR-GECO2 in b ( n = 36 spikes from 3 neurons). ( d ) Fluorescence image of the 4 C . elegans expressing NIR-GECO2-T2A-HO1 in AVA neurons (under flp-18 promoter) and CoChR-GFP in ASH neurons (under sra-6 promoter). The merged image is shown. Imaging conditions: NIR-GECO2, λ ex = 640-nm laser light, λ em = 685/40; GFP, λ ex = 488-nm laser light, λ ex = 527/50 nm. ( e ) Individual traces of NIR-GECO2 fluorescence in an AVA neuron under blue light illumination (20 mW/mm 2 , λ ex = 488-nm laser light, 100 ms; blue bars). The underlying data for b , c , and e can be found in . NIR, near-infrared; SD, standard deviation.
Virus, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/php+eb+aav+syn+jgcamp7s+wpre/pm41519964-232-8-23?v=Addgene+inc
Average 94 stars, based on 1 article reviews
virus - by Bioz Stars, 2026-08
94/100 stars
  Buy from Supplier

Image Search Results


a , Experimental diagram of fiber photometry and optogenetic stimulation. ChrimsonR-expressing CST axons were optically stimulated and calcium responses of jGCaMP7s-expressing Chx10 + interneurons were recorded at C5 and C7. Bottom, experimental timeline. b , Representative images of jGCaMP7s (green) and ChrimsonR-tdTomato expression (red) in the cervical cord at day 28 post-stroke. Bottom, a magnified view of the dotted area. Scale bars, 100 µm. c , Representative optically-evoked calcium responses of V2a interneurons at C5 (left) and C7 (right) levels in control (black) and stroke mice at day 28 (blue) and 56 (red). The solid lines and shaded areas represent the average and standard deviation of fluorescence changes, respectively. d , Peak amplitude of calcium responses. N = 6, 5 and 7 mice for control, day 28, and day 56, respectively; one-way ANOVA followed by Tukey’s test, *** p < 0.001 and **** p < 0.0001. e , Representative kymographs of calcium responses evoked by optical stimulation at C5 and C7 in controls and at day 28 and 56 post-stroke (50 trials/one mouse). The green bars and the dotted lines indicate the timing of photostimulation.

Journal: bioRxiv

Article Title: Scg2 drives reorganization of the corticospinal circuit with spinal premotor interneurons to recover motor function after stroke

doi: 10.1101/2025.01.21.634186

Figure Lengend Snippet: a , Experimental diagram of fiber photometry and optogenetic stimulation. ChrimsonR-expressing CST axons were optically stimulated and calcium responses of jGCaMP7s-expressing Chx10 + interneurons were recorded at C5 and C7. Bottom, experimental timeline. b , Representative images of jGCaMP7s (green) and ChrimsonR-tdTomato expression (red) in the cervical cord at day 28 post-stroke. Bottom, a magnified view of the dotted area. Scale bars, 100 µm. c , Representative optically-evoked calcium responses of V2a interneurons at C5 (left) and C7 (right) levels in control (black) and stroke mice at day 28 (blue) and 56 (red). The solid lines and shaded areas represent the average and standard deviation of fluorescence changes, respectively. d , Peak amplitude of calcium responses. N = 6, 5 and 7 mice for control, day 28, and day 56, respectively; one-way ANOVA followed by Tukey’s test, *** p < 0.001 and **** p < 0.0001. e , Representative kymographs of calcium responses evoked by optical stimulation at C5 and C7 in controls and at day 28 and 56 post-stroke (50 trials/one mouse). The green bars and the dotted lines indicate the timing of photostimulation.

Article Snippet: The following plasmids were generated for AAV production or transfections in culture: for pAAV-Syn-DIO-jGCaMP7s and pAAV-CAG-jGCaMP7s, jGCaMP7s fragment with BamHI/HindIII site derived from pGP-AAV-Syn-jGCaMP7s-WPRE (Addgene, #104487) was subcloned into pAAV-Syn-DIO-MCS, which had multicloning sites (MCS) replacing mCherry in pAAV-Syn-DIO-mCherry (Addgene, #50459), and into pAAV-CAG-tdTomato (Penn vector core; AV-1-PV3365) in a substitution of tdTomato, respectively.

Techniques: Expressing, Control, Standard Deviation, Fluorescence

A) Adult born immature granule cells were silenced using designer receptor exclusively activated by designer drugs (hM4Di), which activates inhibitory Gi-signaling upon stimulation by the ligand, clozapine-N-oxide (CNO). Tamoxifen (TAM) inducible Ascl CreER ;Di F/F mice and Di F/F controls were injected with Tamoxifen (100mg/kg) for 5 consecutive days, and followed by rAAV injection and window implantation 2 weeks later. Head-fixed mice were subsequently trained to run on a featureless belt to find randomly scattered hidden rewards. (Bottom) Mice were tested in different Contexts as in but different olfactory stimuli were delivered in the middle of one of the belt sections (1sec.). First session in context A was imaged as a baseline, then all mice received CNO (5mg/kg) i . p . injections 30 min before running in contexts A’ and B while at home cage. B) A separate cohort of Ascl CreER ;TdTomato F/F mice were injected with TAM followed by AAV-GCaMp7s injection 2 weeks later. AAV injection did not result in differences in genetically targeted iGCs in the injected hemisphere compared to the contralateral hemisphere, for quantification see . C) Spatial firing patterns during 3 sequential exposures to the same and different contexts in Di F/F control mice. The second exposure to Context A is shown as A’. Cells selected based on having a place field in at least one session and are arranged based on maximum events rate in A. Each row across all graphs represents a single cell matched across all contexts. N Cre- =261 cells, 7 mice. D) Spatial firing patterns during 3 sequential exposures to the same and different contexts in Ascl CreER ;Di F/F mice. N Cre+ = 275 cells, 6 mice. E) Top: Similarity of spatial representations of cells with a field in at least one context are measured by calculating Pearson’s correlations of rate maps between sequential exposures to the same (A-A’) or different (A’-B) contexts (top). Bottom: Bar plots represent group data; circles represent per animal data. Interaction F 1,22 = 11.15, ** P =0.0030; Context F 1,22 = 191.9, *** P <0.0001; Genotype F 1,22 = 34.89, ** P <0.0001; 2-way ANOVA. Same context: P Cre- – Cre + = 0.2928; Different context: *** P Cre- – Cre + <0.0001; Tukey’s multiple comparisons test. F) Position decoded by a Bayesian algorithm among cells found in all sessions by training using neural activity in A’ and calculating predicted position in the same (A) or different (B) contexts (i). Line graphs represent median spatial decoder error calculated as the difference between the predicted position in the training data from those in test datasets that is higher in Cre negative Di F/F animals compared to Ascl CreER ;Di F/F when spatial activity from different environments was used to decode position (ii). Di F/F : W = 28, * P Same Ctx – Different Ctx = 0.0156; Ascl CreER ;Di F/F : W = 15, P Same Ctx – Different Ctx = 0.1563; Wilcoxon rank sum test. iii. Discrimination index was calculated as: (Error Different Context - Error Same Context )/ (Error Different Context + Error Same Context ), U =4, * P Cre—Cre+ = 0 . 0 140, Mann-Whitney test. Di F/F : 83.42 ± 5.89, Ascl CreER ;Di F/F : 83 ± 8.68 granule cells matched in test and train sessions. Error bars, ± sem. ( iii, see also )

Journal: bioRxiv

Article Title: Adult born hippocampal granule cells promote pattern separation by bidirectionally modulating the remapping of place and cue cells

doi: 10.1101/2022.12.08.519632

Figure Lengend Snippet: A) Adult born immature granule cells were silenced using designer receptor exclusively activated by designer drugs (hM4Di), which activates inhibitory Gi-signaling upon stimulation by the ligand, clozapine-N-oxide (CNO). Tamoxifen (TAM) inducible Ascl CreER ;Di F/F mice and Di F/F controls were injected with Tamoxifen (100mg/kg) for 5 consecutive days, and followed by rAAV injection and window implantation 2 weeks later. Head-fixed mice were subsequently trained to run on a featureless belt to find randomly scattered hidden rewards. (Bottom) Mice were tested in different Contexts as in but different olfactory stimuli were delivered in the middle of one of the belt sections (1sec.). First session in context A was imaged as a baseline, then all mice received CNO (5mg/kg) i . p . injections 30 min before running in contexts A’ and B while at home cage. B) A separate cohort of Ascl CreER ;TdTomato F/F mice were injected with TAM followed by AAV-GCaMp7s injection 2 weeks later. AAV injection did not result in differences in genetically targeted iGCs in the injected hemisphere compared to the contralateral hemisphere, for quantification see . C) Spatial firing patterns during 3 sequential exposures to the same and different contexts in Di F/F control mice. The second exposure to Context A is shown as A’. Cells selected based on having a place field in at least one session and are arranged based on maximum events rate in A. Each row across all graphs represents a single cell matched across all contexts. N Cre- =261 cells, 7 mice. D) Spatial firing patterns during 3 sequential exposures to the same and different contexts in Ascl CreER ;Di F/F mice. N Cre+ = 275 cells, 6 mice. E) Top: Similarity of spatial representations of cells with a field in at least one context are measured by calculating Pearson’s correlations of rate maps between sequential exposures to the same (A-A’) or different (A’-B) contexts (top). Bottom: Bar plots represent group data; circles represent per animal data. Interaction F 1,22 = 11.15, ** P =0.0030; Context F 1,22 = 191.9, *** P <0.0001; Genotype F 1,22 = 34.89, ** P <0.0001; 2-way ANOVA. Same context: P Cre- – Cre + = 0.2928; Different context: *** P Cre- – Cre + <0.0001; Tukey’s multiple comparisons test. F) Position decoded by a Bayesian algorithm among cells found in all sessions by training using neural activity in A’ and calculating predicted position in the same (A) or different (B) contexts (i). Line graphs represent median spatial decoder error calculated as the difference between the predicted position in the training data from those in test datasets that is higher in Cre negative Di F/F animals compared to Ascl CreER ;Di F/F when spatial activity from different environments was used to decode position (ii). Di F/F : W = 28, * P Same Ctx – Different Ctx = 0.0156; Ascl CreER ;Di F/F : W = 15, P Same Ctx – Different Ctx = 0.1563; Wilcoxon rank sum test. iii. Discrimination index was calculated as: (Error Different Context - Error Same Context )/ (Error Different Context + Error Same Context ), U =4, * P Cre—Cre+ = 0 . 0 140, Mann-Whitney test. Di F/F : 83.42 ± 5.89, Ascl CreER ;Di F/F : 83 ± 8.68 granule cells matched in test and train sessions. Error bars, ± sem. ( iii, see also )

Article Snippet: Mice were unilaterally injected with recombinant adeno-associated virus (rAVV) carrying the GCaMP6s or GCaMP7s transgenes (pAAV.Syn.GCaMP6s.WPRE.SV40 or pGP-AAV-syn-jGCaMP7s-WPRE) purchased from Addgene (viral preps #100843-AAV1, 104487-AAV1) with titers of 1-5×10 12 in dorsal dentate gyrus using a Nanoject syringe (Drummond Scientific, Broomall, PA).

Techniques: Injection, Control, Activity Assay, MANN-WHITNEY

A) ( i ) Ascl CreER ;Di F/F mice were injected with TAM 4-6 weeks later acute slice were prepared (left). A bipolar stimulating electrode was positioned in the lateral perforant path (LPP, the outermost section of the DG molecular layer). Whole cell current clamp recordings were performed on mGCs before (baseline) and during bath application of CNO (5mM). ( ii ) Average evoked synaptic potentials in a representative mGC during baseline (blue) and CNO application (red). AUC indicates area above baseline (depolarization). W = 112, * P Baseline-CNO = 0.0004, Wilcoxon matched pairs signed rank test, N=15 cells from 8 mice. B) Ascl CreER ;TdTomato F/F mice were injected with TAM followed by unilateral AAV-GCaMp7s injection into the dorsal DG 2 weeks later (left). Quantification of TdTomato signal in upper blade of the DG shown in . W = 13, P IR-Day – IR-Week = 0.125, Wilcoxon matched pairs signed rank test. N=5 mice, 3 matched sections/mouse. C) Population vector (PV) correlations of granule cells with significant fields in same contexts at each treadmill position in control (left) and X-IR (right) mice. For calculating PVs, lap-averaged spatial firing rate maps of all tuned cells in the second session of Context A (A’) were correlated with that of the first session of Context A. D) Population vector (PV) correlations of granule cells with significant fields in different contexts at each treadmill position in control (left) and X-IR (right) mice. Lap-averaged spatial firing rate maps of all tuned cells in the second session of Context A were correlated with that of the Context B. E) The effect size of Control - X-IR (Cohen’s d =1.48, r =0.59) and Di F/F - Ascl CreER Di F/F (Cohen’s d =1.37, r =0.56) cohorts were comparable. Further, comparison of the context discrimination index calculated as: (Error Different Context - Error Same Context )/ (Error Different Context + Error Same Context ), in both groups of mice. Interaction F 1,26 = 0.12, P = 0.089; Manipulation F 1,26 = 0.008, P =0.92; Genotype F 1,26 = 112.6, *** P < 0.0001; 2-way ANOVA.

Journal: bioRxiv

Article Title: Adult born hippocampal granule cells promote pattern separation by bidirectionally modulating the remapping of place and cue cells

doi: 10.1101/2022.12.08.519632

Figure Lengend Snippet: A) ( i ) Ascl CreER ;Di F/F mice were injected with TAM 4-6 weeks later acute slice were prepared (left). A bipolar stimulating electrode was positioned in the lateral perforant path (LPP, the outermost section of the DG molecular layer). Whole cell current clamp recordings were performed on mGCs before (baseline) and during bath application of CNO (5mM). ( ii ) Average evoked synaptic potentials in a representative mGC during baseline (blue) and CNO application (red). AUC indicates area above baseline (depolarization). W = 112, * P Baseline-CNO = 0.0004, Wilcoxon matched pairs signed rank test, N=15 cells from 8 mice. B) Ascl CreER ;TdTomato F/F mice were injected with TAM followed by unilateral AAV-GCaMp7s injection into the dorsal DG 2 weeks later (left). Quantification of TdTomato signal in upper blade of the DG shown in . W = 13, P IR-Day – IR-Week = 0.125, Wilcoxon matched pairs signed rank test. N=5 mice, 3 matched sections/mouse. C) Population vector (PV) correlations of granule cells with significant fields in same contexts at each treadmill position in control (left) and X-IR (right) mice. For calculating PVs, lap-averaged spatial firing rate maps of all tuned cells in the second session of Context A (A’) were correlated with that of the first session of Context A. D) Population vector (PV) correlations of granule cells with significant fields in different contexts at each treadmill position in control (left) and X-IR (right) mice. Lap-averaged spatial firing rate maps of all tuned cells in the second session of Context A were correlated with that of the Context B. E) The effect size of Control - X-IR (Cohen’s d =1.48, r =0.59) and Di F/F - Ascl CreER Di F/F (Cohen’s d =1.37, r =0.56) cohorts were comparable. Further, comparison of the context discrimination index calculated as: (Error Different Context - Error Same Context )/ (Error Different Context + Error Same Context ), in both groups of mice. Interaction F 1,26 = 0.12, P = 0.089; Manipulation F 1,26 = 0.008, P =0.92; Genotype F 1,26 = 112.6, *** P < 0.0001; 2-way ANOVA.

Article Snippet: Mice were unilaterally injected with recombinant adeno-associated virus (rAVV) carrying the GCaMP6s or GCaMP7s transgenes (pAAV.Syn.GCaMP6s.WPRE.SV40 or pGP-AAV-syn-jGCaMP7s-WPRE) purchased from Addgene (viral preps #100843-AAV1, 104487-AAV1) with titers of 1-5×10 12 in dorsal dentate gyrus using a Nanoject syringe (Drummond Scientific, Broomall, PA).

Techniques: Injection, Plasmid Preparation, Control, Comparison

(A) Schematic diagram of viral vector ( AAV-TRE-jGCaMP7s ) injection and optical fiber implantation at the SCN in control ( Avp-Cre; Vgat wt/flox ; Vip-tTA ) or Avp-Vgat −/− ( Avp-Cre; Vgat flox/flox ; Vip-tTA ) mice for fiber photometry recording. (B) A representative coronal section of mice with jGCaMP7s expression in SCN VIP neurons. A white dotted square shows the estimated position of the implanted optical fiber. Green, jGCaMP7s; blue, DAPI. Scale bar, 1 mm. (C) Representative plots of the in vivo jGCaMP7s signal of SCN VIP neurons (green: VIP-Ca 2+ ) overlaid with locomotor activity (black) in actograms. Control (Left) and Avp-Vgat −/− (Right) mice were initially housed in LD (LD1 to LD5), then in DD (DD1 to DD13). Gray shading indicates the time when the lights were off. (D) Plots of locomotor activity onset (black), activity offset (gray), VIP-Ca 2+ onset (green), VIP-Ca 2+ offset (light green), and VIP-Ca 2+ midpoint (magenta) of mean ± SEM (left column) and individual (right column) in control and Avp-Vgat −/− mice. Identical marker shapes indicate data from the same animal. (E) High VIP-Ca 2+ duration in LD (LD1-5, left) or in DD (DD6-10, right). (F) Normalized VIP-Ca 2+ daily rhythm profiles in LD (LD1-5, top) or in DD (DD6-10, bottom). In DD, CT12 was determined as the onset of locomotor activity. Red, Avp-Vgat −/− (n = 8); blue, Control ( Avp-Vgat +/− , i.e., Avp-Cre; Vgat wt/flox , n = 5); Values are mean ± SEM. *P < 0.05 by two-tailed Welch’s t-test.

Journal: bioRxiv

Article Title: GABAergic network from AVP neurons to VIP neurons in the suprachiasmatic nucleus sets the activity/rest time of the circadian behavior rhythm

doi: 10.1101/2025.04.28.650944

Figure Lengend Snippet: (A) Schematic diagram of viral vector ( AAV-TRE-jGCaMP7s ) injection and optical fiber implantation at the SCN in control ( Avp-Cre; Vgat wt/flox ; Vip-tTA ) or Avp-Vgat −/− ( Avp-Cre; Vgat flox/flox ; Vip-tTA ) mice for fiber photometry recording. (B) A representative coronal section of mice with jGCaMP7s expression in SCN VIP neurons. A white dotted square shows the estimated position of the implanted optical fiber. Green, jGCaMP7s; blue, DAPI. Scale bar, 1 mm. (C) Representative plots of the in vivo jGCaMP7s signal of SCN VIP neurons (green: VIP-Ca 2+ ) overlaid with locomotor activity (black) in actograms. Control (Left) and Avp-Vgat −/− (Right) mice were initially housed in LD (LD1 to LD5), then in DD (DD1 to DD13). Gray shading indicates the time when the lights were off. (D) Plots of locomotor activity onset (black), activity offset (gray), VIP-Ca 2+ onset (green), VIP-Ca 2+ offset (light green), and VIP-Ca 2+ midpoint (magenta) of mean ± SEM (left column) and individual (right column) in control and Avp-Vgat −/− mice. Identical marker shapes indicate data from the same animal. (E) High VIP-Ca 2+ duration in LD (LD1-5, left) or in DD (DD6-10, right). (F) Normalized VIP-Ca 2+ daily rhythm profiles in LD (LD1-5, top) or in DD (DD6-10, bottom). In DD, CT12 was determined as the onset of locomotor activity. Red, Avp-Vgat −/− (n = 8); blue, Control ( Avp-Vgat +/− , i.e., Avp-Cre; Vgat wt/flox , n = 5); Values are mean ± SEM. *P < 0.05 by two-tailed Welch’s t-test.

Article Snippet: In addition, we modified this plasmid to make an improved version by using an EcoRI-HindIII fragment of this plasmid containing jGCaMP7s sequence to replace an EcoRI-HindIII fragment containing ChrimsonR-mCherry from pAAV-TRE-ChrimsonR-mCherry (Addgene #92207, a gift from Alice Ting)[ ]. pAAV-U6-gGabrb1∼3-EF1α-DIO-mCherry , plasmids for CRISPR-Cas9-mediated Gabrb1∼3 gene disruption, was generated as follows.

Techniques: Plasmid Preparation, Injection, Control, Expressing, In Vivo, Activity Assay, Marker, Two Tailed Test

(A) Schematic diagram of viral vector (AAV- CAG-FLEX-jGCaMP7s and AAV- U6-gGabrb1,2,3-EF1α-DIO-mCherry or AAV- U6-gControl-EF1α-DIO-mCherry ) injection and optical fiber implantation at the SCN in Rosa26-CAG-LSL-SpCas9-2A-EGFP; Vip-ires-Cre mice to generate control or Vip-GABA A R −/− mice for fiber photometry recording. (B) A representative coronal section of mice with jGCaMP7s expression and gRNA-DIO-mCherry expression in SCN VIP neurons. A white dotted square shows the estimated position of the implanted optical fiber. Green, jGCaMP7s; magenta, mCherry. (C) Representative plots of the in vivo jGCaMP7s signal of SCN VIP neurons (green) overlaid with locomotor activity (home-cage activity) (black) in actograms. Control (Left) and Vip-GABA A R −/− (Right) mice were initially housed in LD (LD1 to LD7) and then in DD (DD1 to DD15). Gray shading indicates the time when the lights were off. (D) Plots of locomotor activity onset (black), activity offset (gray), VIP-Ca 2+ onset (green), VIP-Ca 2+ offset (light green), and VIP-Ca 2+ midpoint (magenta) of mean ± SEM (left column) and individual (right column) in control and Vip-GABA A R -/- mice. (E) Normalized VIP-Ca 2+ daily rhythm profiles in LD (LD3-7, top) or in DD (DD5-14,middle and bottom). In DD, circadian time 12 was determined as the onset of locomotor activity and VIP-Ca 2+ circadian time 0 was determined as the onset of VIP-Ca 2+ . (F) High VIP-Ca 2+ duration in LD (LD3-7, left) or in DD (DD5-14, right). (G) High VIP-Ca 2+ duration in LD (LD3-7, left) or in DD (DD5-14, right) calculated with 20% amplitude of the VIP-Ca 2+ profile as the threshold to determine the Ca 2+ onset and offset. Values are mean ± SEM. n = 7 for control, n = 5 for Vip-GABA A R −/− mice. *P < 0.05; **P < 0.01 by two-tailed Student t tests.

Journal: bioRxiv

Article Title: GABAergic network from AVP neurons to VIP neurons in the suprachiasmatic nucleus sets the activity/rest time of the circadian behavior rhythm

doi: 10.1101/2025.04.28.650944

Figure Lengend Snippet: (A) Schematic diagram of viral vector (AAV- CAG-FLEX-jGCaMP7s and AAV- U6-gGabrb1,2,3-EF1α-DIO-mCherry or AAV- U6-gControl-EF1α-DIO-mCherry ) injection and optical fiber implantation at the SCN in Rosa26-CAG-LSL-SpCas9-2A-EGFP; Vip-ires-Cre mice to generate control or Vip-GABA A R −/− mice for fiber photometry recording. (B) A representative coronal section of mice with jGCaMP7s expression and gRNA-DIO-mCherry expression in SCN VIP neurons. A white dotted square shows the estimated position of the implanted optical fiber. Green, jGCaMP7s; magenta, mCherry. (C) Representative plots of the in vivo jGCaMP7s signal of SCN VIP neurons (green) overlaid with locomotor activity (home-cage activity) (black) in actograms. Control (Left) and Vip-GABA A R −/− (Right) mice were initially housed in LD (LD1 to LD7) and then in DD (DD1 to DD15). Gray shading indicates the time when the lights were off. (D) Plots of locomotor activity onset (black), activity offset (gray), VIP-Ca 2+ onset (green), VIP-Ca 2+ offset (light green), and VIP-Ca 2+ midpoint (magenta) of mean ± SEM (left column) and individual (right column) in control and Vip-GABA A R -/- mice. (E) Normalized VIP-Ca 2+ daily rhythm profiles in LD (LD3-7, top) or in DD (DD5-14,middle and bottom). In DD, circadian time 12 was determined as the onset of locomotor activity and VIP-Ca 2+ circadian time 0 was determined as the onset of VIP-Ca 2+ . (F) High VIP-Ca 2+ duration in LD (LD3-7, left) or in DD (DD5-14, right). (G) High VIP-Ca 2+ duration in LD (LD3-7, left) or in DD (DD5-14, right) calculated with 20% amplitude of the VIP-Ca 2+ profile as the threshold to determine the Ca 2+ onset and offset. Values are mean ± SEM. n = 7 for control, n = 5 for Vip-GABA A R −/− mice. *P < 0.05; **P < 0.01 by two-tailed Student t tests.

Article Snippet: In addition, we modified this plasmid to make an improved version by using an EcoRI-HindIII fragment of this plasmid containing jGCaMP7s sequence to replace an EcoRI-HindIII fragment containing ChrimsonR-mCherry from pAAV-TRE-ChrimsonR-mCherry (Addgene #92207, a gift from Alice Ting)[ ]. pAAV-U6-gGabrb1∼3-EF1α-DIO-mCherry , plasmids for CRISPR-Cas9-mediated Gabrb1∼3 gene disruption, was generated as follows.

Techniques: Plasmid Preparation, Injection, Control, Expressing, In Vivo, Activity Assay, Two Tailed Test

(A, B) Top: Representative traces of the jGCaMP7s signal of SCN VIP neurons upon optogenetic stimulation of AVP neurons at various timing in vivo (A, ChrimsonR; B, mCherry-Control). Green traces indicate the fluorescence (F) value at the 470 nm light excitation (F470), Ca 2+ -dependent signal. Magenta traces indicate the fluorescence value at the 415 nm light excitation (F415), Ca 2+ -independent control signal. Red shading indicates the timing of optical stimulation (635 nm, 50 ms pulse, 5 Hz, 120 s). Bottom: Ratio (R) calculated by F470/F415 from the upper traces. The baseline ratio (R 0 ) is the mean R-value of the pre-stimulation period (-30 s - 0 s). ΔR is the difference between the mean R-value of the late phase during the stimulation period (R 1 , 90 s - 120 s, R 1 ) and R 0 . a.u., arbitrary unit. (C, D) Daily rhythms of the baseline ratio (R 0 , open circle) and the ratio during the late phase of stimulation (R 1 , closed circle). Each color represents an individual mouse. C, ChrimsonR; D, mCherry-Control. (E) Comparison of the ΔR/R 0 %. Optogenetic stimulation of SCN AVP neurons increases VIP-Ca 2+ during the night in freely moving mice. n = 6 for ChrimsonR, n = 4 for control. ***P < 0.001 by two-tailed Welch’s t-test. Four out of 6 in ChrimsonR and all control animals are from a previously used cohort .

Journal: bioRxiv

Article Title: GABAergic network from AVP neurons to VIP neurons in the suprachiasmatic nucleus sets the activity/rest time of the circadian behavior rhythm

doi: 10.1101/2025.04.28.650944

Figure Lengend Snippet: (A, B) Top: Representative traces of the jGCaMP7s signal of SCN VIP neurons upon optogenetic stimulation of AVP neurons at various timing in vivo (A, ChrimsonR; B, mCherry-Control). Green traces indicate the fluorescence (F) value at the 470 nm light excitation (F470), Ca 2+ -dependent signal. Magenta traces indicate the fluorescence value at the 415 nm light excitation (F415), Ca 2+ -independent control signal. Red shading indicates the timing of optical stimulation (635 nm, 50 ms pulse, 5 Hz, 120 s). Bottom: Ratio (R) calculated by F470/F415 from the upper traces. The baseline ratio (R 0 ) is the mean R-value of the pre-stimulation period (-30 s - 0 s). ΔR is the difference between the mean R-value of the late phase during the stimulation period (R 1 , 90 s - 120 s, R 1 ) and R 0 . a.u., arbitrary unit. (C, D) Daily rhythms of the baseline ratio (R 0 , open circle) and the ratio during the late phase of stimulation (R 1 , closed circle). Each color represents an individual mouse. C, ChrimsonR; D, mCherry-Control. (E) Comparison of the ΔR/R 0 %. Optogenetic stimulation of SCN AVP neurons increases VIP-Ca 2+ during the night in freely moving mice. n = 6 for ChrimsonR, n = 4 for control. ***P < 0.001 by two-tailed Welch’s t-test. Four out of 6 in ChrimsonR and all control animals are from a previously used cohort .

Article Snippet: In addition, we modified this plasmid to make an improved version by using an EcoRI-HindIII fragment of this plasmid containing jGCaMP7s sequence to replace an EcoRI-HindIII fragment containing ChrimsonR-mCherry from pAAV-TRE-ChrimsonR-mCherry (Addgene #92207, a gift from Alice Ting)[ ]. pAAV-U6-gGabrb1∼3-EF1α-DIO-mCherry , plasmids for CRISPR-Cas9-mediated Gabrb1∼3 gene disruption, was generated as follows.

Techniques: In Vivo, Control, Fluorescence, Comparison, Two Tailed Test

(A) Schematic diagram of viral vector injection at the SCN in Avp-Cre; Vip-tTA mice for slice recording of VIP-Ca 2+ and optogenetic stimulation of AVP neurons. (B) A representative coronal section of mice with ChrimsonR expression in AVP neurons (left) and jGCaMP7s expression in VIP neurons (right). White dotted squares indicate the estimated position of the optical fiber. (C) Average traces of the jGCaMP7s signal of SCN VIP neurons with (ChrimsonR, blue) or without (Control, purple) optogenetic stimulation of AVP neurons at ZT22. Red shading indicates the timing of optical stimulation (617 nm, 40 ms pulse, 10 Hz, 120 s). (D) The mean ΔF/F 0 % values during the last 30 s of optogenetic stimulation (90 s – 120 s in C). Values are mean ± SEM. n=3 for control (mCherry) mice, n = 6 for ChrimsonR mice. *P < 0.05 by two-tailed Welch’ s t-test. (E) Average trace of jGCaMP7s signal in VIP neurons after administration of gabazine (GABA A R antagonist, 10 μM) via bath perfusion. The green shading indicates when Gabazine was present. (F) The mean ΔF/F 0 % value during the last 60 s of gabazine application (180 s –240 s in E). Baseline is the mean ΔF/F 0 % values 2 min prior to gabazine application (-120 – 0 s). Values are mean ± SEM. n = 6. *P < 0.05 by two-tailed paired t-test. (G) Average traces of the jGCaMP7s signal of SCN VIP neurons upon optogenetic stimulation of AVP neurons at ZT22 without (ChrimsonR, blue) or with (ChrimsonR + Gabazine, red) gabazine application. Red shading indicates the timing of optical stimulation (617 nm, 40 ms pulse, 10 Hz, 120 s). The ChrimsonR traces in (C, blue) and (G, blue) are identical. (H) The mean ΔF/F 0 % values during the last 30 s of optogenetic stimulation (90 s – 120 s in G). Values are mean ± SEM. n = 6. *P < 0.05 by two-tailed paired t-test.

Journal: bioRxiv

Article Title: GABAergic network from AVP neurons to VIP neurons in the suprachiasmatic nucleus sets the activity/rest time of the circadian behavior rhythm

doi: 10.1101/2025.04.28.650944

Figure Lengend Snippet: (A) Schematic diagram of viral vector injection at the SCN in Avp-Cre; Vip-tTA mice for slice recording of VIP-Ca 2+ and optogenetic stimulation of AVP neurons. (B) A representative coronal section of mice with ChrimsonR expression in AVP neurons (left) and jGCaMP7s expression in VIP neurons (right). White dotted squares indicate the estimated position of the optical fiber. (C) Average traces of the jGCaMP7s signal of SCN VIP neurons with (ChrimsonR, blue) or without (Control, purple) optogenetic stimulation of AVP neurons at ZT22. Red shading indicates the timing of optical stimulation (617 nm, 40 ms pulse, 10 Hz, 120 s). (D) The mean ΔF/F 0 % values during the last 30 s of optogenetic stimulation (90 s – 120 s in C). Values are mean ± SEM. n=3 for control (mCherry) mice, n = 6 for ChrimsonR mice. *P < 0.05 by two-tailed Welch’ s t-test. (E) Average trace of jGCaMP7s signal in VIP neurons after administration of gabazine (GABA A R antagonist, 10 μM) via bath perfusion. The green shading indicates when Gabazine was present. (F) The mean ΔF/F 0 % value during the last 60 s of gabazine application (180 s –240 s in E). Baseline is the mean ΔF/F 0 % values 2 min prior to gabazine application (-120 – 0 s). Values are mean ± SEM. n = 6. *P < 0.05 by two-tailed paired t-test. (G) Average traces of the jGCaMP7s signal of SCN VIP neurons upon optogenetic stimulation of AVP neurons at ZT22 without (ChrimsonR, blue) or with (ChrimsonR + Gabazine, red) gabazine application. Red shading indicates the timing of optical stimulation (617 nm, 40 ms pulse, 10 Hz, 120 s). The ChrimsonR traces in (C, blue) and (G, blue) are identical. (H) The mean ΔF/F 0 % values during the last 30 s of optogenetic stimulation (90 s – 120 s in G). Values are mean ± SEM. n = 6. *P < 0.05 by two-tailed paired t-test.

Article Snippet: In addition, we modified this plasmid to make an improved version by using an EcoRI-HindIII fragment of this plasmid containing jGCaMP7s sequence to replace an EcoRI-HindIII fragment containing ChrimsonR-mCherry from pAAV-TRE-ChrimsonR-mCherry (Addgene #92207, a gift from Alice Ting)[ ]. pAAV-U6-gGabrb1∼3-EF1α-DIO-mCherry , plasmids for CRISPR-Cas9-mediated Gabrb1∼3 gene disruption, was generated as follows.

Techniques: Plasmid Preparation, Injection, Expressing, Control, Two Tailed Test

(A) Schematic diagram of viral vector injection at the SCN in Avp-Cre mice for slice recording of SCN non-AVP cellular Ca 2+ and optogenetic stimulation of AVP neurons. (B) A representative coronal section of mice with jGCaMP7s expression in non-AVP cells (left) and ChrimsonR expression in AVP neurons (right). White dotted squares indicate the estimated position of the optical fiber. The red rectangles in (B) indicate the regions of the enlarged images (C). (C) Left: Representative coronal section of Avp-Cre mice with jGCaMP7s expression in non-AVP cells (2.6 μm/pixl). White outline indicates the regions considered as the SCN. Right: Representative pixel-level heat maps (20.8 μm/pixel) showing the jGCaMP7s signal from non-AVP cells in response to the optogenetic stimulation of AVP neurons at ZT22. Optical stimulation was applied from time 0 to 120 s. Blue and red squares on the maps indicate regions (4 x 4 pixels) considered as the middle and ventral regions, respectively, for the subsequent analyses. (D) Representative responses of the jGCaMP7s signals in the middle (left) and ventral (right) regions to the optogenetic activation of AVP neurons. Red shading indicates the timing of optical stimulation (617 nm, 40 ms pulse, 10 Hz, 120 s). M P0 and M T0 are the highest (peak) and lowest (trough) ΔF/F 0 %-value of the pre-stimulation (-60 s ∼ 0 s) in the middle region, respectively. M P and M T are the highest and lowest ΔF/F 0 %-value during the stimulation (0 s ∼ 120 s). Correspondingly, V P0 , V T0 , V P , and V T refer to the respective points in the ventral region. (E) Mean responses of the meddle and ventral SCN regions to the optogenetic activation of AVP neurons. Values are mean ± SEM. n = 5. *P < 0.05 by two-tailed Welch’ s t-test.

Journal: bioRxiv

Article Title: GABAergic network from AVP neurons to VIP neurons in the suprachiasmatic nucleus sets the activity/rest time of the circadian behavior rhythm

doi: 10.1101/2025.04.28.650944

Figure Lengend Snippet: (A) Schematic diagram of viral vector injection at the SCN in Avp-Cre mice for slice recording of SCN non-AVP cellular Ca 2+ and optogenetic stimulation of AVP neurons. (B) A representative coronal section of mice with jGCaMP7s expression in non-AVP cells (left) and ChrimsonR expression in AVP neurons (right). White dotted squares indicate the estimated position of the optical fiber. The red rectangles in (B) indicate the regions of the enlarged images (C). (C) Left: Representative coronal section of Avp-Cre mice with jGCaMP7s expression in non-AVP cells (2.6 μm/pixl). White outline indicates the regions considered as the SCN. Right: Representative pixel-level heat maps (20.8 μm/pixel) showing the jGCaMP7s signal from non-AVP cells in response to the optogenetic stimulation of AVP neurons at ZT22. Optical stimulation was applied from time 0 to 120 s. Blue and red squares on the maps indicate regions (4 x 4 pixels) considered as the middle and ventral regions, respectively, for the subsequent analyses. (D) Representative responses of the jGCaMP7s signals in the middle (left) and ventral (right) regions to the optogenetic activation of AVP neurons. Red shading indicates the timing of optical stimulation (617 nm, 40 ms pulse, 10 Hz, 120 s). M P0 and M T0 are the highest (peak) and lowest (trough) ΔF/F 0 %-value of the pre-stimulation (-60 s ∼ 0 s) in the middle region, respectively. M P and M T are the highest and lowest ΔF/F 0 %-value during the stimulation (0 s ∼ 120 s). Correspondingly, V P0 , V T0 , V P , and V T refer to the respective points in the ventral region. (E) Mean responses of the meddle and ventral SCN regions to the optogenetic activation of AVP neurons. Values are mean ± SEM. n = 5. *P < 0.05 by two-tailed Welch’ s t-test.

Article Snippet: In addition, we modified this plasmid to make an improved version by using an EcoRI-HindIII fragment of this plasmid containing jGCaMP7s sequence to replace an EcoRI-HindIII fragment containing ChrimsonR-mCherry from pAAV-TRE-ChrimsonR-mCherry (Addgene #92207, a gift from Alice Ting)[ ]. pAAV-U6-gGabrb1∼3-EF1α-DIO-mCherry , plasmids for CRISPR-Cas9-mediated Gabrb1∼3 gene disruption, was generated as follows.

Techniques: Plasmid Preparation, Injection, Expressing, Activation Assay, Two Tailed Test

( a ) Left, fluorescent image of neurons expressing NLS-jGCaMP7s (λ ex = 488-nm laser light, λ em = 527/50 nm). Right, fluorescent image of neurons expressing NIR-GECO2-T2A-HO1 (λ ex = 640-nm laser light, λ em = 685/40 nm). Representative of more than 3 worms, both under tag-168 promoter. Scale bar, 50 μm. ( b ) Fluorescence traces of NLS-jGCaMP7s (top) and NLS-NIR-GECO2 (bottom) in response to the stimulation of microfluidic containing 200 mM NaCl. Solid lines represent averaged data from 3 neurons. Shaded areas are shown as SD. Triangles on the top of the traces indicate the time points of stimulation (20 seconds for each stimulation). ( c ) Quantitative fluorescence changes of NLS-jGCaMP7s and NLS-NIR-GECO2 in b ( n = 36 spikes from 3 neurons). ( d ) Fluorescence image of the 4 C . elegans expressing NIR-GECO2-T2A-HO1 in AVA neurons (under flp-18 promoter) and CoChR-GFP in ASH neurons (under sra-6 promoter). The merged image is shown. Imaging conditions: NIR-GECO2, λ ex = 640-nm laser light, λ em = 685/40; GFP, λ ex = 488-nm laser light, λ ex = 527/50 nm. ( e ) Individual traces of NIR-GECO2 fluorescence in an AVA neuron under blue light illumination (20 mW/mm 2 , λ ex = 488-nm laser light, 100 ms; blue bars). The underlying data for b , c , and e can be found in . NIR, near-infrared; SD, standard deviation.

Journal: PLoS Biology

Article Title: Improved genetically encoded near-infrared fluorescent calcium ion indicators for in vivo imaging

doi: 10.1371/journal.pbio.3000965

Figure Lengend Snippet: ( a ) Left, fluorescent image of neurons expressing NLS-jGCaMP7s (λ ex = 488-nm laser light, λ em = 527/50 nm). Right, fluorescent image of neurons expressing NIR-GECO2-T2A-HO1 (λ ex = 640-nm laser light, λ em = 685/40 nm). Representative of more than 3 worms, both under tag-168 promoter. Scale bar, 50 μm. ( b ) Fluorescence traces of NLS-jGCaMP7s (top) and NLS-NIR-GECO2 (bottom) in response to the stimulation of microfluidic containing 200 mM NaCl. Solid lines represent averaged data from 3 neurons. Shaded areas are shown as SD. Triangles on the top of the traces indicate the time points of stimulation (20 seconds for each stimulation). ( c ) Quantitative fluorescence changes of NLS-jGCaMP7s and NLS-NIR-GECO2 in b ( n = 36 spikes from 3 neurons). ( d ) Fluorescence image of the 4 C . elegans expressing NIR-GECO2-T2A-HO1 in AVA neurons (under flp-18 promoter) and CoChR-GFP in ASH neurons (under sra-6 promoter). The merged image is shown. Imaging conditions: NIR-GECO2, λ ex = 640-nm laser light, λ em = 685/40; GFP, λ ex = 488-nm laser light, λ ex = 527/50 nm. ( e ) Individual traces of NIR-GECO2 fluorescence in an AVA neuron under blue light illumination (20 mW/mm 2 , λ ex = 488-nm laser light, 100 ms; blue bars). The underlying data for b , c , and e can be found in . NIR, near-infrared; SD, standard deviation.

Article Snippet: The genes of NIR-GECO2, NIR-GECO2G, HO1, CoChR, and jGCaMP7s for expression in C . elegans were codon-optimized using SnapGene codon-optimization tool and synthesized by GenScript.

Techniques: Expressing, Fluorescence, Imaging, Standard Deviation

( a ) Fluorescence intensity of NLS-jGCaMP7s and NIR-GECO2 in C . elegans neurons at resting state. Fluorescence was normalized to the same excitation intensity ( n = 132 ROIs from 5 worms; data are shown as mean ± SD) ( b ) SBR of NLS-jGCaMP7s and NIR-GECO2 in neurons of C . elegans at resting state ( n = 132 ROIs from 5 worms; data are shown as mean ± SD). SBR was obtained via dividing the fluorescence intensity from neurons by the averaged autofluorescence from the intestine area. ( c ) SNR of NLS-jGCaMP7s and NLS-NIR-GECO2 quantified from spontaneously spiking neurons (n = 78 ROIs from 4 worms; data are shown as mean ± SD). SNR was calculated by dividing the fluorescence change associated with a spike by the SD of the baseline fluorescence over the 2-second period immediately before the spike. ( d ) The ratio of SBR NIR-GECO2 to SBR NLS-jGCaMP7s at different imaging depths ( n = 5 worms; data are shown as mean ± SD). ( e ) The ratio of SNR NLS-NIR-GECO2 to SNR NLS-jGCaMP7s at different imaging depths ( n = 4 worms; data are shown as mean ± SD). NIR-GECO2 (without NLS) and NLS-jGCaMP7s were used for the experiments in a , b , and d ; NLS-NIR-GECO2 and NLS-jGCaMP7s were used for the experiments in c and e . The underlying data for a – e can be found in . ROI, region of interest; SBR, signal-to-background ratio; SD, standard deviation; SNR, signal-to-noise ratio.

Journal: PLoS Biology

Article Title: Improved genetically encoded near-infrared fluorescent calcium ion indicators for in vivo imaging

doi: 10.1371/journal.pbio.3000965

Figure Lengend Snippet: ( a ) Fluorescence intensity of NLS-jGCaMP7s and NIR-GECO2 in C . elegans neurons at resting state. Fluorescence was normalized to the same excitation intensity ( n = 132 ROIs from 5 worms; data are shown as mean ± SD) ( b ) SBR of NLS-jGCaMP7s and NIR-GECO2 in neurons of C . elegans at resting state ( n = 132 ROIs from 5 worms; data are shown as mean ± SD). SBR was obtained via dividing the fluorescence intensity from neurons by the averaged autofluorescence from the intestine area. ( c ) SNR of NLS-jGCaMP7s and NLS-NIR-GECO2 quantified from spontaneously spiking neurons (n = 78 ROIs from 4 worms; data are shown as mean ± SD). SNR was calculated by dividing the fluorescence change associated with a spike by the SD of the baseline fluorescence over the 2-second period immediately before the spike. ( d ) The ratio of SBR NIR-GECO2 to SBR NLS-jGCaMP7s at different imaging depths ( n = 5 worms; data are shown as mean ± SD). ( e ) The ratio of SNR NLS-NIR-GECO2 to SNR NLS-jGCaMP7s at different imaging depths ( n = 4 worms; data are shown as mean ± SD). NIR-GECO2 (without NLS) and NLS-jGCaMP7s were used for the experiments in a , b , and d ; NLS-NIR-GECO2 and NLS-jGCaMP7s were used for the experiments in c and e . The underlying data for a – e can be found in . ROI, region of interest; SBR, signal-to-background ratio; SD, standard deviation; SNR, signal-to-noise ratio.

Article Snippet: The genes of NIR-GECO2, NIR-GECO2G, HO1, CoChR, and jGCaMP7s for expression in C . elegans were codon-optimized using SnapGene codon-optimization tool and synthesized by GenScript.

Techniques: Fluorescence, Imaging, Standard Deviation